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- Elabscience
- 湖北武汉
- E-AB-F1026E
- 2025年07月13日
- FCM
- Syrian Hamster
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Syrian Hamster IgG
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
APC
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1026E
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Syrian Hamster
- 应用范围：
FCM
- 抗体英文名：
APC Anti-Mouse CD28 Antibody[37.51]_货号:E-AB-F1026E
- 抗体名：
APC Anti-Mouse CD28 Antibody流式抗体[37.51]_货号:E-AB-F1026E
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥660.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥1620.0 |
货号：E-AB-F1026E
| Background |
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
|
| Alternate Names |
T-cell-specific surface glycoprotein CD28;Cd28;CD28;
|
| Swissprot |
P31041
|
| Gene ID |
12487
|
| Clone No |
37.51
|
| Host |
Syrian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Syrian Hamster IgG
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>
分钟收集细胞； 5. 将上一步离心得到的细胞沉淀，重悬至扩增培养基中，并进行计数。 1.3 细胞刺激（次日） 扩增培养基成分：RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28（克隆号：37.51） + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml（本次实验为 2×10^6/ml，该浓度偏大）； 2. 从 4°C 冰箱取出前一天包被的板子，弃掉包被液，无菌PBS洗涤3遍； 3. 每孔加入 500μl 细胞悬液，放置于 37°C 含 5% CO2 细胞
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
with 30 U/ml rIL-2 in a suitable culture plate or tissue culture flask. 2) Add Dynabeads Mouse T-Activator CD3/CD28 at a bead-to-cell ratio of 1:1. 3) Add 30 U/ml rIL-2. 4) Incubate in a humidified CO2 incubator at 37
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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