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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG2a, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
PE/Cyanine5
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-F1001G
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Mouse
- 应用范围:
FCM
- 抗体英文名:
PE/Cyanine5 Anti-Human CD3 Antibody[OKT-3]_货号:E-AB-F1001G
- 抗体名:
PE/Cyanine5 Anti-Human CD3 Antibody流式抗体[OKT-3]_货号:E-AB-F1001G
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥1872.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥744.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥2880.0 |
货号:E-AB-F1001G
| Background |
CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
|
| Alternate Names |
T-cell surface glycoprotein CD3 epsilon chain;CD3E;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;
|
| Swissprot |
P07766
|
| Gene ID |
916
|
| Clone No |
OKT-3
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE/Cyanine5 Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE/Cyanine5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验自2012年第一篇引用文献问世,至2023年8月,Elabscience已协助各地科研工作者发表超13,000篇SCI文献,包括行业顶/级期刊Nature Medicine, Nature, Cell, Signal Transduction and Targeted Therapy, Circulation, Molecular Cancer, Immunity, Advanced Materials等,总影响因子(IF)59472.59,其中IF≥30的文献超30篇,最高影响因子为66.849,发文单位包含北京大学、中国科学技术大学、浙江大学、斯坦福大学、耶鲁大学、柏林夏里特大学等国内外各大高校。
每年引用文献数量持续增长,保持逐年30%+高增长率,可以预见,Elabscience产品将帮助更多科研工作者发表高质量SCI论文。
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1 ml 2.4 中准备好的 Biotin Elution Buffer,让液流完全进入胶体后孵育 2 min,加入 9 ml 的 Biotin Elution Buffer; 1.13 对于从全血中分选目的细胞,再加入 10 ml 的 Biotin Elution Buffer; 1.14 300 g 转速离心 10 分钟,收集洗脱目的细胞。 流程图: 2. 实验数据 以下流式图为从 7.5 ml 全血中,选出 CD3 + T 细胞的数据。选后细胞以 CD3 -APC (OKT
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法: 货号 名称
: 由此步骤开始,流出的溶液包含目标细胞,请使用新的试剂管收集!首先,加入 1 ml 的 biotin 洗脱液,孵育 2 min。其次,加入 10 ml biotin 洗脱液,洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液,洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中,选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC(OKT-3) 及 CD45-PE(HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞
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