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Cytokeratin 18 antibody [N2C2]

, Internal
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX105624
  • 2025年07月12日
  • WB, ICC/IF, IHC-P, IP
  • Rabbit
  • Human, Mouse, Dog
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Dog

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX105624

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P, IP

    • 浓度

      0.54 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      Cytokeratin 18

    • 抗体英文名

      Cytokeratin 18 antibody [N2C2], Internal

    • 抗体名

      Cytokeratin 18 抗体 [N2C2], Internal

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.

    产品细节图片2

    Sample(30 μg of whole cell lysate)
    A:H1299
    B:Hep G2(GTX27900)
    7.5% SDS PAGE
    GTX105624 diluted at 1:3000

    产品细节图片3

    Immunofluorescence analysis of PFA-fixed HeLa, using Cytokeratin 18 (GTX105624) antibody at 1:200 dilution.

    产品细节图片4

    Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18(GTX105624) antibody at 1:100 dilution.
    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片5

    Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded dog kidney.
    Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片6

    Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded dog liver.
    Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片7

    Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX105624) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    产品细节图片8

    Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis.
    Sample: Paraffin-embedded dog malignant carcinoma.
    Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片9

    Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded dog colon.
    Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片10

    Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm by immunofluorescent analysis.
    Sample: MDCK cells were fixed in 4% PFA at RT for 15 min.
    Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.

    产品细节图片11

    Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded dog intestine.
    Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Wuputra K et al., Int J Mol Sci 2022 (PMID:35887129)
    Yu HI et al., J Pharm Biomed Anal 2018 (PMID:30114613)
    Xing C et al., Neoplasia 2014 (PMID:25425963)
    Luni C et al., Nat Methods 2016 (PMID:27088312)
    Sundararaman B et al., Mol Cell 2016 (PMID:26990993)
    相关实验
    • secondary antibody review -- data from 99 publications

      Signaling 18   IgG   Alexa Fluor 488 immunocytochemistry 1:2000     18   IgG   Alexa Fluor 568 immunocytochemistry 1:2000     19 donkey IgG   HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat

    • RUVKUN Antibody Staining Protocol revised 10/29/90

      amts!, don't use up sample) with 1% toluidine blue. Rinse several times in buffer B. Compare two samples at 50X under dissecting scope. If animals are " open" , + RNAse animals will have very little staining of internal tissues. ANTIBODY INCUBATIONS

    • (共享)免疫组化常用标记抗体

      negative, while the bile ducts are positive.CAM 5.2- Monoclonal antibody against low M.W. cytokeratin (including CK8 and CK18).- Source: Becton-Dickinson.- Dilution 1:8.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    Cytokeratin 18 antibody [N2C2], Internal
    ¥1700 - 4000