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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Ku70. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101848
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Ku70
- 抗体英文名:
Ku70 antibody [N1N3]
- 抗体名:
Ku70 抗体 [N1N3]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101848 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Ku70(GTX101848) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunofluorescence analysis of PFA-fixed HeLa, using Ku70(GTX101848) antibody at 1:500 dilution.
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文献和实验Chiang JH et al., Hum Exp Toxicol 2013 (PMID:24064905)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
Mol Cell:杨薇等揭示 DNA-PK 的激活机制以及自磷酸化对 NHEJ 通路的重要作用
变化,收紧 N-HEAT 环,最终与 M-HEAT 的 MH3 亚结构域和 FAT 的 FR2 亚结构域相互作用;FAT 结构域内的相邻 HEAT 重复序列之间则发生相反反向的扭转和拉升,激活态复合物 IV 中 FR2 与 NH1 相互作用; 最终,Ku70/80 和 DNA 末端将 DNA 损伤信号通过 DNA-PKcs 亚基上的 HEAT 重复序列的「彩虹圈式」构象变化传递到激酶结构域,PRD 发生 115° 外翻,解除激酶活性中心抑制态,暴露 DNA-PK 的底物结合口袋,催化 DNA-PK
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