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ILK antibody [N1C1]

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月16日
  • WB, IHC-P, IHC-Wm, IP, IHC
  • Rabbit
  • Human, Mouse, Rat, Zebrafish
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat, Zebrafish

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX101691

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, IHC-P, IHC-Wm, IP, IHC

    • 浓度

      0.8 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      ILK

    • 抗体英文名

      ILK antibody [N1C1]

    • 抗体名

      ILK 抗体 [N1C1]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片2

    Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

    产品细节图片3

    ILK antibody [N1C1] detects ILK protein at cytoplasm in mouse kidney by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse kidney.
    ILK antibody [N1C1] (GTX101691) diluted at 1:500.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片4

    ILK antibody [N1C1] detects ILK protein by western blot analysis.
    A. 30 μg Neuro2A whole cell extract
    B. 30 μg C8D30 whole cell extract
    C. 30 μg NIH-3T3 whole cell extract
    D. 30 μg Raw 264.7 whole cell extract
    E. 30 μg C2Cl2 whole cell extract
    10 % SDS-PAGE
    ILK antibody [N1C1] (GTX101691) dilution: 1:1000

    产品细节图片5

    Sample(30 μg of whole cell lysate)
    A:A431(GTX27909)
    7.5% SDS PAGE
    GTX101691 diluted at 1:500

    产品细节图片6

    ILK antibody [N1C1] immunoprecipitates ILK protein in IP experiments.
    IP samples: HeLa whole cell extract
    A. 30 μg HeLa whole cell extract
    B. Control with 4 μg of preimmune Rabbit IgG
    C. Immunoprecipitation of ILK protein by 4 μg ILK antibody [N1C1] (GTX101691)
    10 % SDS-PAGE
    The immunoprecipitated ILK protein was detected by ILK antibody [N1C1] (GTX101691) diluted at 1:500.
    [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

    产品细节图片7

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

    产品细节图片8

    Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

    产品细节图片9

    Sample (30 μg of whole cell lysate)
    A: zebrafish muscle
    10% SDS PAGE
    GTX101691 diluted at 1:1000

    产品细节图片10

    Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1] (GTX101691) at 1:300 dilution.

    产品细节图片11

    Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ILK antibody [N1C1] (GTX101691) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Chen YL et al., Sci Rep 2021 (PMID:33436772)
    Wantoch von Rekowski K et al., Biomolecules 2019 (PMID:31779287)
    Yen YC et al., Oncotarget 2015 (PMID:26540630)
    Huang CY et al., Mol Neurobiol 2014 (PMID:24771044)
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    文献支持
    ILK antibody [N1C1]
    ¥1700 - 4000