Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat, Zebrafish
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX101691
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, IHC-P, IHC-Wm, IP, IHC
浓度:
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
ILK
抗体英文名:
ILK antibody [N1C1]
抗体名:
ILK 抗体 [N1C1]
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.
ILK antibody [N1C1] detects ILK protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ILK antibody [N1C1] (GTX101691) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ILK antibody [N1C1] detects ILK protein by western blot analysis. A. 30 μg Neuro2A whole cell extract B. 30 μg C8D30 whole cell extract C. 30 μg NIH-3T3 whole cell extract D. 30 μg Raw 264.7 whole cell extract E. 30 μg C2Cl2 whole cell extract 10 % SDS-PAGE ILK antibody [N1C1] (GTX101691) dilution: 1:1000
Sample(30 μg of whole cell lysate) A:A431(GTX27909) 7.5% SDS PAGE GTX101691 diluted at 1:500
ILK antibody [N1C1] immunoprecipitates ILK protein in IP experiments. IP samples: HeLa whole cell extract A. 30 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of ILK protein by 4 μg ILK antibody [N1C1] (GTX101691) 10 % SDS-PAGE The immunoprecipitated ILK protein was detected by ILK antibody [N1C1] (GTX101691) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.
Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Sample (30 μg of whole cell lysate) A: zebrafish muscle 10% SDS PAGE GTX101691 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1] (GTX101691) at 1:300 dilution.
Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ILK antibody [N1C1] (GTX101691) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Application Reference
Chen YL et al., Sci Rep 2021 (PMID:33436772)
Wantoch von Rekowski K et al., Biomolecules 2019 (PMID:31779287)
Yen YC et al., Oncotarget 2015 (PMID:26540630)
Huang CY et al., Mol Neurobiol 2014 (PMID:24771044)