![Nanog antibody [N3C3]](https://img1.dxycdn.com/2023/0711/255/2344116916935858761-14.jpg)
- ￥1700 - 4000
- GeneTex
- 美国
- GTX100863
- 2025年07月13日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA
- Rabbit
- Human, Mouse, Ceratotherium simum
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Ceratotherium simum
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100863
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Nanog
- 抗体英文名：
Nanog antibody [N3C3]
- 抗体名：
Nanog 抗体 [N3C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
![Nanog antibody [N3C3]](https://img1.dxycdn.com/p/s14/2024/0410/033/0677551891969387771.jpg)
NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% PFA/culture medium at RT for 30 min.
Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.
![Nanog antibody [N3C3]](https://img1.dxycdn.com/p/s14/2024/0410/201/0253847971969387771.jpg)
Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Nanog antibody [N3C3]](https://img1.dxycdn.com/p/s14/2024/0410/898/0443106461969387771.jpg)
Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
![Nanog antibody [N3C3]](https://img1.dxycdn.com/p/s14/2024/0410/221/4454632302969387771.jpg)
Immunofluorescence analysis of PFA-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.
![Nanog antibody [N3C3]](https://img1.dxycdn.com/p/s14/2024/0410/942/0230678652969387771.jpg)
ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL.
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