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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100748
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Syk
- 抗体英文名:
Syk antibody [N2C2], Internal
- 抗体名:
Syk 抗体 [N2C2], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
SYK antibody [N2C2], Internal detects SYK protein by western blot analysis.
A. 30 μg mouse BMDM (bone marrow-derived macrophage) cells
B. 30 μg mouse Syk null cells
10% SDS-PAGE
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed A431, using SYK(GTX100748) antibody at 1:200 dilution.
Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 μg of SYK antibody [N2C2] (GTX100748).
Western blot analysis was performed using SYK antibody [N2C2] (GTX100748).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SYK antibody [N2C2], Internal detects SYK protein at cytosol on rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SYK antibody detects SYK protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Jia Y et al., FASEB J 2018 (PMID:29401630)
Tomoyuki Mukai et al., J Bone Miner Res 2014 (PMID:24916406)
Jia Y et al., Cell Death Dis 2019 (PMID:30674869)
Preparation and Assay of Acetylcholinesterase Antibody
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Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Analysis of Antibody-Antigen Interactions Using Surface Plasmon Resonance
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