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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考说明书
- 亚型:
请参考说明书
- 形态:
液体
- 保存条件:
Aliquot and store at -20ºC
- 克隆性:
请参考说明书
- 标记物:
请参考说明书
- 适应物种:
human/mouse/rat等
- 宿主:
rabbit/mouse
- 应用范围:
ELISA,WB,IHC等
- 浓度:
请参考说明书
- 靶点:
请参考说明书
- 抗体英文名:
VEGFB 167/186 antibody
- 抗体名:
VEGFB 167/186 antibody
- 规格:
25μg
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文献和实验is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
IMMUNOHISTOCHEMISTRY on PARAFFIN OR CRYOSECTIONS
DAY ONE Paraffin sections: 1) Deparaffinize: Xylene , 3x, 5 min each 2) Rehydrate: 100% EtOH, 2x, 5 min each 95% EtOH, 2x, 5 min each 80% EtOH, 1x, 5 min 50% EtOH, 1x, 5 min Proceed to Antigen Retrieval below (step 3) Cryo sections
Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab)
Day 1 1. Add formaledehyde directly to tissue culture media to a final concentration of 1%. We generally use 2 x 107 cells per antibody per timepoint. Fewer cells can be used but usually results in a lower signal to noise ratio.
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