- ￥1700 - 4000
- GeneTex
- 美国
- GTX109306
- 2025年07月10日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein corresponding to human DNase I.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX109306
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.19 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNase I
- 抗体英文名：
DNase I antibody [N1C3]
- 抗体名：
DNase I 抗体 [N1C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNase I antibody [N1C3] (GTX109306) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DNase I protein stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DNase I stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Ueki M et al., Sci Rep 2019 (PMID:31541133)
Lam C et al., Biotechnol Prog 2017 (PMID:28127892)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
DNase I Footprint Analysis of Protein‐DNA Binding
I (DNase I) protection mapping, or footprinting, is a valuable technique for locating the specific binding sites of proteins on DNA. The basis of this assay is that bound protein protects the phosphodiester backbone of DNA from DNase I?catalyzed hydrolysis
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
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