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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Materials Stock Acrylamide: (30%T:0.8%C) 30% by weight of acrylamide 0.8% by weight of N,N'-bis-methylene acrylamide Separation Gel (Final Concentrations) 10% acrylamide
Agarose Gel Electrophoresis for the Separation of DNA Fragments
. The volume of the buffer should not be greater than 1/3 of the capacity of the flask. 2) Add running buffer to the agarose-containing flask. Swirl to mix. The most common gel running buffers are TAE (40 mM Tris-acetate, 1 mM EDTA) and TBE (45 mM Tris-borate
oligonucleotides that require a small gel pore size for retardation. Separation of Proteins and Nucleic Acids Proteins are amphoteric compounds; their nett charge therefore is determined by the pH of the medium in which they are suspended. In a solution
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