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文献和实验Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
Zone electrophoresis is the basic mode of capillary electrophoresis, and it allows separation of ions having different charge-to-size ratios. Carbohydrates having ionic groups can also be the objects of separation by this mode
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
bromophenyl blue [BioRad] (~0.1% in H2O)per ml of 5X buffer (this may inhibit the binding of some proteins )Store buffer at -70 degrees.--------------------------------------------------------------------------------Gel shift reactions are performed as follows
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
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