Mouse/Human/Rat Phospho-MEF2A
/ MEF2 (Ser408) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F910- $800
- LSBio
- To be determined
- LS-F910
- 美国
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Mouse/Human/Rat Phospho-MEF2A / MEF2 (Ser408) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F910
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Product Description
LS-F910 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MEF2A / MEF2 in samples of Cell Lysates and Nuclear Lysates. It is based upon a DNA Binding Phosphorylation assay principle.Specifications
TypeDNA Binding Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
MEF2A / MEF2
Reactivity
Mouse, Human, Rat
Specificity
The MEF2A (Phospho-Ser408) DNA-Binding ELISA Kit detects endogenous levels of MEF2A only when phosphorylated at Ser408.
Manual
Intended Sample Types
Cell Lysates, Nuclear Lysates
Format
12 x 8-Well Microstrips
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Control Lysates
- Control Oligonucleotides
- Extraction Buffers
- Detection Antibodies
- Wash Buffers
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About MEF2A / MEF2
Q02078 NM_005587 NP_005578.2
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DNA-Binding Phosphorylation ELISA Platform Overview
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
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