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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
NEBNext® Small RNA 多样本文库制备试剂盒 - Illumina® (检索引物1-48) 收藏
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
NEBNext® Small RNA 多样本文库制备试剂盒 - Illumina® (检索引物 1-48) 中包含接头，引物，酶及缓冲液，可将 small RNAs 转化为可用于 Illumina 测序平台的包含 index 序列的二代测序文库。新颖的工作流程经过优化，能够最大程度上减少接头二聚体的形成，所制备的文库产量高，多样性好。
每个建库试剂盒的组分必须通过严格的质控标准，每组试剂都通过构建含 index 的 small RNA 文库并在 Illumina 平台测序进行功能验证。
NEBNext® Small RNA 多样本文库制备试剂盒 - Illumina® (检索引物1-48)
试剂盒组分：
| 本试剂盒提供以下组分：
|
||
| 储存温度 (°C)
|
浓度
|
|
| NEBNext 3´ Ligation Reaction Buffer
|
-20
|
2X
|
| NEBNext 3´ Ligation Enzyme Mix
|
-20
|
|
| NEBNext 3´ SR Adaptor for Illumina
|
-20
|
|
| NEBNext 5´ SR Adaptor for Illumina
|
-20
|
|
| NEBNext 5´ Ligation Reaction Buffer
|
-20
|
|
| NEBNext 5´ Ligation Enzyme Mix
|
-20
|
|
| NEBNext SR RT Primer for Illumina
|
-20
|
|
| NEBNext First Strand Synthesis Reaction Buffer
|
-20
|
5X
|
| ProtoScript® II Reverse Transcriptase
|
-20
|
200,000 units/ml
|
| Murine RNase Inhibitor
|
||
| LongAmp™ Taq 2X Master Mix
|
-20
|
2X
|
| NEBNext Index 1-48 Primers for Illumina
|
-20
|
|
| NEBNext SR Primer for Illumina
|
-20
|
|
| Gel Loading Dye, Blue
|
25
|
6X
|
| Quick-Load pBR322 DNA-MspI Digest
|
-20
|
50 μg/ml
|
| DNA Gel Elution Buffer
|
-20
|
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Assay of superoxide dismutase activity
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