- ￥200 - 400
- NEB
- 美国
- #E7546L
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
NEBNext® Ultra™ II End Repair/dA-Tailing Module 收藏
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II End Ligation Module (NEB #E7595), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA.
This module is compatible with Illumina workflows and with some Oxford Nanopore MinION™workflows.
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Ultra II End Prep Enzyme Mix | -20 | |
| NEBNext Ultra II End Prep Reaction Buffer | -20 |
Description
The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II End Ligation Module (NEB #E7595), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA.
This module is compatible with Illumina workflows and with some Oxford Nanopore MinION™workflows.
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Ultra II End Prep Enzyme Mix | -20 | |
| NEBNext Ultra II End Prep Reaction Buffer | -20 |
Properties and Usage
Storage Temperature
-20°C
南京赛泓瑞生物科技有限公司是专业从事生化试剂、分子生物学试剂、实验耗材及实验仪器研发、销售、服务为一体的专业性生物科技公司。本着服务科学的理念，赛泓瑞建立了生命科学研究工具的研发、生产、销售及技术服务平台，现已有数万余种产品，并每年不断开发新产品，为广大的生命科学研究人员、医学、药学、农业、环保等的科研人员提供科研工具性产品及技术服务.
经营范围：抑制剂、ELISA试剂盒、原代细胞和细胞株、化学试剂、蛋白、抗体、分子试剂和试剂盒。
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x volume of filtrate) to each well. Seal with plastic sealing tape and mix. Incubate
A general protocol for staining cell for cytometry analysis
V-FITC (Cat. No. 65874X, 65874H) or Annexin V-Biotin (Cat. Nos. 65872X, 65872H). 3. Via-Probe™ (Cat. No. 34321X): A convenient ready-to-use solution of the nucleic acid dye 7-AAD. Recommended for use in parallel with Annexin V-PE (Cat
Cleavage Efficiency Close to the Termini of PCR Fragments
When designing PCR experiments in which the synthesized DNA fragment is to be subsequently digested with a RE, it is very important to determine how many extra nucleotides should be added to the 5’-end of the PCR primer
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