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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
EnGen® Spy dCas9 (SNAP-tag®)
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
EnGen® Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity (1,2). EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag® allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
Product Source
EnGen® Spy dCas9 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
Reagents Supplied
The following reagents are supplied with this product
| Store at (°C) | Concentration | |
| NEBuffer™ 3.1 | -20 | 10X |
Description
EnGen® Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity (1,2). EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag® allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
Product Source
EnGen® Spy dCas9 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
Reagents Supplied
The following reagents are supplied with this product
| Store at (°C) | Concentration | |
| NEBuffer™ 3.1 | -20 | 10X |
Advantages and Features
Applications
- Localization of DNA targets in vivo
- Target Enrichment
Properties and Usage
Reaction Conditions
1X NEBuffer 3.1
Incubate at 37°C
1X NEBuffer™ 3.1:
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 μg/ml BSA
pH 7.9 @ 25°C
Storage Temperature
-20°C
Storage Conditions
300 mM NaCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.4 @ 25°C
Related Products
Companion Products
- EnGen® sgRNA Synthesis Kit, S. pyogenes
- HiScribe™ T7 Quick High Yield RNA Synthesis Kit
- SNAP-Vista® Green
- SNAP-Capture Magnetic Beads
Notes
- 1..20 µM is equal to ~3.6 mg/ml and 1uM is equal to ~ 0.18 mg/ml EnGen® Spy dCas9.
- 2. This product is compatible with Diluent Buffer B: 300 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 500 µg/ml BSA and 50% glycerol. (pH 7.4 @ 25°C).
References
- Qi, LS., et. al. (2013). Cell. 152 (5), 1173-83.
- Qi, LS., et. al. (2013). Cell. 154 (2), 442-51.
南京赛泓瑞生物科技有限公司是专业从事生化试剂、分子生物学试剂、实验耗材及实验仪器研发、销售、服务为一体的专业性生物科技公司。本着服务科学的理念，赛泓瑞建立了生命科学研究工具的研发、生产、销售及技术服务平台，现已有数万余种产品，并每年不断开发新产品，为广大的生命科学研究人员、医学、药学、农业、环保等的科研人员提供科研工具性产品及技术服务.
经营范围：抑制剂、ELISA试剂盒、原代细胞和细胞株、化学试剂、蛋白、抗体、分子试剂和试剂盒。
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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
extension cycle at 72 ˚C for 5 min. PCR reactions were performed in Gene Amp® PCR System 480 (Perkin-Elmer, Norwalk, CT, USA). DNA samples of normal subjects (blood donors) were included as well as negative control (with no DNA sample) in each reaction.
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
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