- $990
- LSBio
- 0.156 - 10 ng/ml
- LS-F34604
- 美国
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.156 - 10 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Human MAP2K1 / MKK1 / MEK1 ELISA Kit (Sandwich ELISA) - LS-F34604
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Product Description
LS-F34604 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MAP2K1 / MKK1 / MEK1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MAP2K1 / MKK1 / MEK1 as low as 0.094 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
MAP2K1 / MKK1 / MEK1
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.094 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MAP2K1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MAP2K1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP - Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAP2K1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAP2K1 can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About MAP2K1 / MKK1 / MEK1
Q02750 NM_002755 NP_002746.1
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Sandwich ELISA Platform Overview
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
Western时，您会得到一个量化值，哪些组分占多少。Sampler Kit包含细胞质MEK1/2、线粒体凋亡诱导因子(AIF)、组蛋白H3和细胞骨架波形蛋白的兔单克隆抗体，以及山羊抗兔IgG。 研究人员也可利用试剂盒中的建议，或搜索文献，组装自己的Western blot或ELISA试剂盒。不过Cormier也提醒，并非每个抗体都有着它应有的特异性。 下游的分析 与超速离心相比，基于试剂盒的细胞分级分离方法快速又方便，也适合多个下游应用，如EMSA、报告基因分析、酶活
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
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