- $675
- LSBio
- To be determined
- LS-F406
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Rat JAG1 / Jagged 1 ELISA Kit (Custom ELISA)
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Product Description
LS-F406 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat JAG1 / Jagged 1. It is based upon a Custom assay principle.Specifications
TypeCustom ELISA kit
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Target
JAG1 / Jagged 1
Reactivity
Rat
Specificity
The antibody pair provided in this kit recognizes rat JAG1 / Jagged 1.
Manual
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
To be determined
Sensitivity
To be determined
Storage
Short term: 4°C; Long term: see manual.
Kit Components
Typical components include:
- Microplate - Item A
- Wash Buffer Concentrate (20x) - Item B
- Standards - Item C
- Assay Diluent A - Item D
- Assay Diluent B - Item E
- Biotinylated Detection Antibody
- HRP-Streptavidin concentrate - Item G
- TMB Substrate
- Stop Solution - Item I
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 hours at room temperature or overnight at 4°C.
- Add 100 µl prepared Detection Antibody to each well.
- Incubate 1 hour at room temperature.
- Add 100 µl prepared Streptavidin solution.
- Incubate 45 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well.
- Read at 450 nm immediately.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About JAG1 / Jagged 1
P78504 NM_000214 NP_000205.1
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