- ￥5600
- GeneTex
- 美国
- GTX22768
- 2025年07月16日
- WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, Gel supershift assays
- Mouse
- Human, Mouse, Rat, Yeast, Rabbit, Sheep, Guinea pig, Xenopus, Primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Partially purified rat GR.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Yeast, Rabbit, Sheep, Guinea pig, Xenopus, Primate
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rat
- 目录编号：
GTX22768
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, Gel supershift assays
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Using enzymatic digestion analysis, this antibody reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
- 抗体英文名：
Glucocorticoid Receptor antibody [BuGR2]
- 抗体名：
Glucocorticoid Receptor 抗体 [BuGR2]
- 规格：
100 μg
FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% PFA
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].
ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% PFA
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% PFA
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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文献和实验Chemical Mutational Analysis of Glucocorticoid Receptor
This chapter describes a chemical method for mutational analysis of the human glucocorticoid receptor (hGR) cDNA. Corticosteroid-resistant (CR) asthma is associated with in vitro and in vivo defects in mononuclear cell function
High-Yield Purification of Functionally Active Glucocorticoid Receptor
The glucocorticoid receptor (GR) resides in the cytoplasm as a complex with chaperones. Upon ligand association, GR shuttles into the nucleus, and binds to its hormone response elements to control expression of particular genes
by the human glucocorticoid receptor α (hGRα), a ligand-dependent transcription factor ubiquitously expressed in almost all tissues and cells. The molecular mechanisms of hGRα action involve (a) binding to glucocorticoids, (b) cytoplasmic to nuclear
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