- ￥5300
- GeneTex
- 美国
- GTX79485
- 2025年07月16日
- WB, ELISA, Sandwich ELISA
- Rat
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-6
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX79485
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
WB, ELISA, Sandwich ELISA
- 浓度：
1.01 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IL5
- 抗体英文名：
IL5 antibody [5A10]
- 抗体名：
IL5 抗体 [5A10]
- 规格：
500 μg
Sandwich ELISA analysis of serial diluted mouse IL-5 recombinant protein using IL5 antibody
Coating : GTX79485 IL5 antibody [5A10] in duplicate at 1, 2, 4, 5, 7, and 9 μg/mL
Detection : GTX79486 IL5 antibody [39D10] (Biotin) (0.5 μg/mL)
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文献和实验【讨论】Innate immunity: 'Natural helper' cells identified
from lymphoid progenitors3 and lymphoid tissue inducer cells4. These cells proliferate in response to IL2 and produce large amounts of TH2 cytokines such as IL5, IL6 and IL13. IL5 and IL6 regulate B-cell antibody production and self-renewal of B1 cells
ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
Neutralizing Bioassay Protocols
describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. In general, the cytokine bioassay protocols are modified to pre-incubate the cytokine of interest with the specific neutralizing antibody prior to addition
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