- ￥4700
- GeneTex
- 美国
- GTX80144
- 2025年07月16日
- ICC/IF, IHC-Fr, FACS, IP, Functional Assay
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Mouse CTL clone V4 cells
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX80144
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
ICC/IF, IHC-Fr, FACS, IP, Functional Assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
- 抗体英文名：
CD4 antibody [GK1.5] (Azide Free)
- 抗体名：
CD4 抗体 [GK1.5] (Azide Free)
- 规格：
100 μg
FACS analysis of mouse splenocytes using GTX80144 CD4 antibody [GK1.5] (Azide Free).
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文献和实验Chi-Shuan Fan et al., Cells 2022 (PMID:35053345)
Qizhi Ma et al., Precis Clin Med 2021 (PMID:35693216)
Fu Y et al., Mol Carcinog 2019 (PMID:31397502)
Huang CP et al., Cytotherapy 2017 (PMID:28818454)
Miller M et al., J Immunol 2014 (PMID:24623133)
Das S et al., Proc Natl Acad Sci U S A 2016 (PMID:27799535)
Liu YJ et al., PLoS One 2015 (PMID:26325187)
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Th17 Polarization of Mouse CD4 Cells
;g/ml). Incubate at 37 °C for 2 hours or 4 °C over night. A septically decant antibody solution from the plate. Wash plate 3 times with sterile PBS. Discard liquid. 2) Plate CD4 cells at 1x106/ml. Culture cells for 3 days in presence
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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