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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX80252
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
IHC-Fr, FACS, ELISA, Functional Assay
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). The antibody 87G blocks interaction of HLA-G with inhibitory receptors.,We do not recommend use of this product for Mouse,Rat.
- 抗体英文名:
HLA-G antibody [87G] (Low Endotoxin)
- 抗体名:
HLA-G 抗体 [87G] (Low Endotoxin)
- 规格:
100 μg
Effect on cytolytical activity of human polyclonal NK cells on M8 cells transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6). Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis.
Blocking antibodies -
Column 1-2 : None
Column 3 : Isotype mouse IgG2a control
Column 4 : GTX80252 anti-human HLA-G (87G) purified
Column 5 : anti-human HLA-G (87G) F(ab’)2 fragment
Column 6 : anti-human HLA-G (MEM-G/9)
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文献和实验Yang S et al., Cytotherapy 2020 (PMID:32811747)
Bacterial endotoxin is probably the most common significant contaminant that might be found in antibody preparations. It is found ubiquitously in normal environments but its pyrogenic effects in vivo can be lethal. It is absolutely vital
Purification of Endotoxin Free mAbs
. If you have larger volumes, or your antibody is present at low concentrations/poor yields, we find it useful to concentrate the supernatants by circulating them through the same type of hollow fibre dialysis cartridges we use for cell growth, allowing the slight
Recombinant Immunotoxins with Low Endotoxins for Clinical and Animal Studies
. coli ). As the endotoxin came from E. coli cells and is harmful to animals, it is important to produce the RITs with low endotoxin. This section describes the protocols to produce RITs containing low level of endotoxins.
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