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- 钦诚生物
- 上海
- QC25-03889
- 2025年07月08日
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一年
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- 规格：
250ML
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文献和实验High efficiency LiAc transformation
Tris-HCl, pH 7.5 1 mM EDTA 0.1M LiAc PLATE solution 40% PEG 3350 0.1M LiAc 10 mM Tris-HCl, pH 7.5 1 mM EDTA COMPETENCY: Inoculate a single colony into 100 ml YPD (or selective media if necessary
Preparation of mycoplasma chromosomal DNA in TE
, more proteinase K can be added). 5. Add 1/5 (100 ul) volume of lysis buffer and sharply invert 1-2 times until the solution becomes clear. Then add 100 ul more lysis buffer. 6. Incubate at 37 C overnight. 7. Add equal volume (700ul) of equilibrated phenol
Methods and Software in NGS for TE Analysis
The recent development of next-generation sequencing (NGS) technologies allowed various authors to imagine, test, and validate new approaches for TE analysis, in their nature, type, activity, or quantity. In this chapter, we describe briefly
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