- ￥390
- 钦诚生物
- 上海
- QC25-03851
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温
- 保质期：
一年
- 供应商：
钦诚生物
- 规格：
250ML
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
may not be necessary for all tissues and shorter fixation times may be preferable as X-gal staining may be decreased by lengthy fixation. Embed tissue in OCT (Miles) or gelatin/sucrose mounting medium and freeze on metal chucks cooled in liquid N2
ml This solution has to be prepared Fresh or made into 0.4ml aliquots and stored in the deep freeze. C) Veronal-Acetate Buffer Stock Sodium Acetate (3H20) 3.88g Sodium Barbitone 5.88g Distilled water 200ml D
Tissue sectioning: Cryostat sectioning method
% sucrose in PBS overnight or in 7.5% porcine gelatin (300 Bloom) 15% sucrose in PBS for 6 h at 37oC. * Embed in 15% sucrose/7.5% gelatin, chill to 4oC (you can hold the samples here for a few days). * Remove sucrose/gelatin solution and add Tissue
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