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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The immunogen corresponding to a region within amino acids 2406 and 2419 of mouse RIF1.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX48737
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RIF1
- 抗体英文名:
RIF1 antibody
- 抗体名:
RIF1 抗体
- 规格:
50 μg
Western blot using GeneTex's Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 μg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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文献和实验Skrajna A et al., Nucleic Acids Res 2020 (PMID:32658293)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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