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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human Galectin 1 Recombinant protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX116411
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IP
- 浓度:
1.48 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Galectin 1
- 抗体英文名:
Galectin 1 antibody
- 抗体名:
Galectin 1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunofluorescence analysis of methanol-fixed A431, using Galectin 1(GTX116411) antibody at 1:200 dilution.
Galectin 1 antibody detects Galectin 1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
15% SDS-PAGE
Galectin 1 antibody (GTX116411) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 μg of Galectin 1 antibody (GTX116411) 15% SDS-PAGE The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (GTX116411) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Sample (30 μg of whole cell lysate)
A: BCL-1
15% SDS PAGE
GTX116411 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Sample (30 μg of whole cell lysate)
A: NCI-H929
15% SDS PAGE
GTX116411 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% PFA at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
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文献和实验Lin LH et al., Electrophoresis 2014 (PMID:24723343)
Fan CY et al., Electrophoresis 2013 (PMID:24115172)
Paz H et al., J Exp Clin Cancer Res 2018 (PMID:29580262)
Mori Y et al., J Biol Chem 2015 (PMID:26342075)
May EW et al., Pharmacol Res 2014 (PMID:25242635)
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