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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human CD86 expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX82769
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 靶点:
CD86
- 抗体英文名:
CD86 antibody [1B3]
- 抗体名:
CD86 抗体 [1B3]
- 规格:
100 μl
WB analysis of L1210 (1) and MOLT-4 (2) cell lysate using GTX82769 CD86 antibody [1B3].
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文献和实验Elabscience|巨噬细胞极化总失败?RAW264.7/THP-1/BMDM/hMDM四大模型避坑指南与实操全攻略
16/32 Antibody,轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer轻轻吹打混匀,150 × g离心3 min,弃上清; (6)加入200 μL Cell Staining Buffer重悬细胞沉淀,上机检测。 图1. Raw264.7细胞极化培养流程 2. Raw264.7细胞向M1型巨噬细胞极化结果 图
Incorporation of Immunomodulators into Plasmid DNA Vaccines
. These may include cytokines, chemokines, costimulatory molecules, or hemopoietic growth factors (see ref. 1 ). The choice of immunomodulatory molecule reflects the polarization of the immune response (Th1 and cell-mediated or Th2 and antibody) selected to eliminate
Elabscience | BV2细胞M1极化造模与流式检测,这份Protocol请收好
Buffer(E-CK-A107)或PBS缓冲液重悬细胞沉淀,调整细胞密度为1-10×106个/mL; (3)取100 μL细胞悬液,每组加入2 μL Purified Anti-Mouse CD16/32 Antibody(E-AB-F0997A),轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer(E-CK-A107)轻轻
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