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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of c-Yes (aa10-193) expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83048
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 靶点:
c-Yes
- 抗体英文名:
c-Yes antibody [2F3E6]
- 抗体名:
c-Yes 抗体 [2F3E6]
- 规格:
100 μl
WB analysis of truncated YES1-His recombinant protein (1) and full-length GFP-YES1(aa1-543) transfected COS7 cell lysate (2) using GTX83048 YES1 antibody [2F3E6].
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文献和实验of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Both polyclonal and monoclonal
Generation of Antibody Molecules Through Antibody Engineering
Twenty-five years ago, Georges K�hler and C�sar Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics ( 1 ). However, in spite
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
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