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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human recombinant protein of human NPR3 (NP_000899) produced in HEK293T cell.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX84015
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS
- 浓度:
0.84 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NPR-C
- 抗体英文名:
NPR-C antibody [11B5]
- 抗体名:
NPR-C 抗体 [11B5]
- 规格:
100 μl
WB analysis of HEK293T cells transfected with NPR-C plasmid (Right) or empty vector (Left) for 48 hrs using GTX84015 NPR-C antibody [11B5].
Loading : 5 ug per lane
IHC-P analysis of prostate carcinoma tissue using GTX84015 NPR-C antibody [11B5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
ICC/IF analysis of COS7 cells transiently transfected with NPR-C plasmid using GTX84015 NPR-C antibody [11B5].
IHC-P analysis of human ovary adenocarcinoma tissue using GTX84015 NPR-C antibody [11B5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of HEK293T cells transfected with either NPR-C plasmid(Red) or empty vector control plasmid(Blue) using GTX84015 NPR-C antibody [11B5].
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文献和实验性: 可同时检测重组或天然的人 sCD11b 。 不与 人其它细胞因子有交叉反应。 3. 重复性:板内、板见变异系数均小于10%。 注意事项 1. 以上标准孔及待 测 样品均建议做复孔,每次测定应同时 做 标准曲线。 2. 洗涤过程 很关键。洗涤不充分将导致精确度误差及 OD 值错误地升高。 3. 板条开封后剩余板条要再封好,保持 板条干燥 。 4. 本试剂盒宜置 4 o C冰箱
Generation of Antibody Molecules Through Antibody Engineering
Twenty-five years ago, Georges K�hler and C�sar Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics ( 1 ). However, in spite
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
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