- ￥7300
- GeneTex
- 美国
- GTX84785
- 2025年07月13日
- WB, ICC/IF, IHC-P, FACS
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX84785
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS
- 浓度：
0.87 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
BTK
- 抗体英文名：
BTK antibody [10E10]
- 抗体名：
BTK 抗体 [10E10]
- 规格：
100 μl
IHC-P analysis of human liver tissue using GTX84785 BTK antibody [10E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
WB analysis of various samples using GTX84785 BTK antibody [10E10].
Loading : 10 ug per lane
Dilution : 1:200
WB analysis of HEK293T cells transfected with BTK plasmid (Right) or empty vector (Left) for 48 hrs using GTX84785 BTK antibody [10E10].
Loading : 5 ug per lane
FACS analysis of monocytes from non-XLA or XLA patients using GTX84785 BTK antibody [10E10].
ICC/IF analysis of HT29 cells using GTX84785 BTK antibody [10E10].
WB analysis of various cell lines using GTX84785 BTK antibody [10E10].
Loading : 10 ug per lane
Dilution : 1:200
ICC/IF analysis of COS7 cells transiently transfected with BTK plasmid using GTX84785 BTK antibody [10E10].
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文献和实验Kwak SC et al., Nutrients 2020 (PMID:33081167)
RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
. The first is to Dialyze the coupled antibody against PBS for 24 hours with 3 changes of PBS. Doing this will not change concentration of original antibody (recommended for antibody with a concentration less than 1mg/mL). The second way is to use a PD-10 column. Using
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