- ￥7300
- GeneTex
- 美国
- GTX84814
- 2025年07月05日
- WB, IP, ELISA
- Mouse
- Human, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full-length protein expressed in 293T cell transfected with human BRAF expression vector
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX84814
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IP, ELISA
- 浓度：
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
B-Raf
- 抗体英文名：
B-Raf antibody [4B2]
- 抗体名：
B-Raf 抗体 [4B2]
- 规格：
100 μl
Sandwich ELISA analysis of BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using GTX84820 B-Raf antibody [4A5] (capture antibody) at 5ug/ml and HRP conjugated GTX84814 B-Raf antibody [4B2] (detection antibody) at 0.03ug/ml.
WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84814 B-Raf antibody [4B2].
Loading : 5 ug per lane
IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84814 B-Raf antibody [4B2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
WB analysis of various cell lines using GTX84814 B-Raf antibody [4B2].
Loading : 35 ug per lane
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文献和实验Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
meningococci, there seems to be a correlation between SBA activity and protection (1 ), whereas OP has been less well-studied. This function may have a supplementary or even major role in protection against group B meningococci.
Buffer and save the flowthrough.(12) Wash with 6 ml Buffer A, 6 ml Buffer B, and 6 ml Buffer A. Hold at room temperature for 30 minutes.(13) Wash with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C.(14) To purify
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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