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Rb (phospho Ser
249) antibodyThe antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains serine 249 and threonine 252 (based on Swiss Protein database, accession number P06400).
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse
12 months from the shipping date of the product.
Human
GTX24788
Primary Antibodies
Available
GeneTex
Rabbit
WB
Batch dependent (Please refer to the vial label for the specific concentration.)
Rb (phospho Ser249)
Rb (phospho Ser249) antibody
Rb (phospho Ser249) 抗体
50 μl
WB analysis of whole cell extracts (30 μg lysate) of Jurkat (Lane 1), Serum Starved Jurkat (Lane 2), Serum Starved for overnight following by serum release (Lane 3), and Jurkat treated for 15 min with 25 ng/ml of Calyculin A (lane 4) using GTX24788 Rb (phospho Ser249) antibody.
Dilution : 1:500-1:2000
WB (peptide competition) analysis of Jurkat cells in high growth phase using GTX24788 Rb (phospho Ser249) antibody prior incubated with a generic phosphoserine-containing peptide (Lane 2), a generic phosphothreonine- containing peptide (Lane 3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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