- ￥5600
- GeneTex
- 美国
- GTX24788
- 2025年07月10日
- WB
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains serine 249 and threonine 252 (based on Swiss Protein database, accession number P06400).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24788
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Rb (phospho Ser249)
- 抗体英文名：
Rb (phospho Ser249) antibody
- 抗体名：
Rb (phospho Ser249) 抗体
- 规格：
50 μl
WB analysis of whole cell extracts (30 μg lysate) of Jurkat (Lane 1), Serum Starved Jurkat (Lane 2), Serum Starved for overnight following by serum release (Lane 3), and Jurkat treated for 15 min with 25 ng/ml of Calyculin A (lane 4) using GTX24788 Rb (phospho Ser249) antibody.
Dilution : 1:500-1:2000
WB (peptide competition) analysis of Jurkat cells in high growth phase using GTX24788 Rb (phospho Ser249) antibody prior incubated with a generic phosphoserine-containing peptide (Lane 2), a generic phosphothreonine- containing peptide (Lane 3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Generation and Application of Phospho-specific Antibodies for p53 and pRB
of phosphoproteins. We have improved methods for generation of phosphorylation site-specific antibodies and have successfully obtained antibodies for the analysis of most of the phosphorylation sites on p53 and RB proteins.
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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