- ￥4000
- GeneTex
- 美国
- GTX76469
- 2025年07月09日
- FACS
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
C57BL/10 spleen cell enriched for T lymphocytes
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX76469
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
FACS
- 浓度：
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Mouse CD11b
- 抗体英文名：
CD11b antibody [M1/70.15] (Biotin)
- 抗体名：
CD11b 抗体 [M1/70.15] (Biotin)
- 规格：
100 μg
Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE (GTX76475)
Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b (GTX76473)
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文献和实验Zhang Z et al., Neurobiol Dis 2017 (PMID:29274431)
Wang HT et al., Journal of Food and Drug Analysis 2017 (Epub)
Yang LC et al., Evid Based Complement Alternat Med 2013 (PMID:24191166)
Koutarou Nakamura et al., PLoS Biol 2021 (PMID:34014921)
Wang HT et al., J Food Drug Anal 2018 (PMID:29567238)
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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