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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant mouse IFN gamma.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
Biotin
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX75118
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
FACS, ELISA
- 浓度:
0.5 mg/ml
- 靶点:
Interferon gamma
- 抗体英文名:
Interferon gamma antibody [XMG1.2] (Biotin)
- 抗体名:
Interferon gamma 抗体 [XMG1.2] (Biotin)
- 规格:
100 μg
FACS analysis of C57Bl/6 splenocytes stimulated with PMA and Ionomycin stained APC anti-mouse CD3e antibody, followed by intracellular staining with GTX75118 Interferon gamma antibody [XMG1.2] (Biotin).
Right panel : Co-stained with APC anti-mouse CD3e antibody and GTX75118
Left panel : Co-stained with APC anti-mouse CD3e antibody and Biotin Rat IgG1 isotype control
Antibody amount : 0.25 μg
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
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