- ￥4300
- GeneTex
- 美国
- GTX70141
- 2025年07月13日
- WB, ICC/IF, IHC-P, IP
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full-length recombinant human DNA Ligase I protein
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70141
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNA ligase I
- 抗体英文名：
DNA ligase I antibody [10H5]
- 抗体名：
DNA ligase I 抗体 [10H5]
- 规格：
100 μl
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DNA ligase I stained by DNA ligase I antibody [10H5] (GTX70141) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: DNA ligase I stained by DNA ligase I antibody [10H5] (GTX70141) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (GTX70141) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献和实验Horton JK et al., DNA Repair (Amst) 2013 (PMID:23871146)
Prasad R et al., Nucleic Acids Res 2012 (PMID:23042675)
Song W et al., J Biol Chem 2007 (PMID:17561505)
Windhofer F et al., J Cell Physiol 2007 (PMID:17492771)
Wang H et al., Cancer Res 2005 (PMID:15899791)
Wei L et al., Nat Commun 2021 (PMID:33707452)
Wei L et al., Nat Microbiol 2020 (PMID:32152586)
Tsai YC et al., J Nucleic Acids 2017 (PMID:28116145)
Yuan Y et al., Nucleic Acids Res 2015 (PMID:26350212)
Masaoka A et al., PLoS One 2013 (PMID:23826138)
Asagoshi K et al., DNA Repair (Amst) 2010 (PMID:20006562)
Guo Z et al., Nucleic Acids Res 2009 (PMID:19336415)
Wang W et al., J Biol Chem 2006 (PMID:16731526)
Site-Directed Mutagenesis of Antibody-Variable Regions
has an average error rate of 0.8% (6 ,7 ). In this chapter, we described an efficient method for mutagenesis of cloned antibody fragments in which mutant oligonucleotides are utilized to initiate the synthesis of a phosphorothioate nucleotide containing DNA
Electrophoretic Mobility Shift Assays for ProteinDNA Complexes Involved in DNA Repair
damaged by ultraviolet radiation or the anticancer drug cisplatin. Ku, XRCC4/Ligase IV, and DNA–PKcs, which are involved in the repair of DNA double-strand breaks by nonhomologous end joining, assemble in complexes at DNA ends. This chapter
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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