Full-length recombinant human DNA Ligase I protein
亚型:
IgG1
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX70141
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, IHC-P, IP
浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
DNA ligase I
抗体英文名:
DNA ligase I antibody [10H5]
抗体名:
DNA ligase I 抗体 [10H5]
规格:
100 μl
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.DNA ligase I stained by DNA ligase I antibody [10H5] (GTX70141) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.Green: DNA ligase I stained by DNA ligase I antibody [10H5] (GTX70141) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (GTX70141) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.