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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide: DYKDDDDK conjugated to KLH
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX18230
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
DDDDK tag
- 抗体英文名:
DDDDK tag antibody [F-tag-01]
- 抗体名:
DDDDK tag 抗体 [F-tag-01]
- 规格:
100 μg
ICC/IF analysis of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope using GTX18230 DDDDK tag antibody [F-tag-01].
Panel A : anti-DDDDK antibody (Red)
Panel B : DAPI (blue)
Panel C : Merged
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文献和实验Brown S et al., J Neurosci 2019 (PMID:31399534)
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag(Cleavage Under Targets and Tagmentation)是蛋白-DNA 互作的一大革新技术,它不需要使用甲醛交联以及免疫共沉淀,而是通过针对靶蛋白(如转录因子、染色质重塑蛋白)的抗体和 Protein A/G 的介导,使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头,经 PCR 扩增后即可形成用于高通量测序的文库(见图 1 )。CUT &Tag 技术具有细胞投入量低、信噪
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag?seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing
Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
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