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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Full length protein (Mouse).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Biotin
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX19597
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
WB, FACS
- 浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Recognizes a non-polymorphic (Mr 25-30kD) determinant on the Thy1.1 and Thy1.2 alloantigens. The CD90 Thy1 antigen is present on thymocytes, peripheral T-cells and some intraepithelial T-lymphocytes of all mouse strains tested.
- 抗体英文名:
CD90 antibody [T24/31] (Biotin)
- 抗体名:
CD90 抗体 [T24/31] (Biotin)
- 规格:
100 μg
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- 作者
- 内容
- 询问日期
文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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