- ￥4300
- GeneTex
- 美国
- GTX28245
- 2025年07月15日
- WB, ICC/IF, IP
- Mouse
- Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rabbit muscle GAPDH
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rabbit
- 目录编号：
GTX28245
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
GAPDH
- 抗体英文名：
GAPDH antibody [6C5]
- 抗体名：
GAPDH 抗体 [6C5]
- 规格：
100 μg
Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A).
Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 oC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
Lane 1: Human GAPDH (1 μg)
Lane 2: GAPDH immunoprecipitated from rat heart tissue extract
Lane 3: Only GTX28245 preincubated with Protein A Sepharose
Lane 4: Only Protein A Sepharose
Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded.
Lane 1: isolated human GAPDH, 0.5 μg
Lane 2: human heart tissue extract
Lane 3: pig heart tissue extract
Lane 4: goat heart tissue extract
Lane 5: bovine heart tissue extract
Lane 6: dog heart tissue extract
Lane 7: mouse heart tissue extract
Lane 8: rat heart tissue extract
Lane 9: rabbit heart tissue extract
Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by:
GTX28245 (green colour)
F-actin microfilaments-bindingdye (red)
DNA-binding dye (dark blue)
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文献和实验Fuerst E et al., Allergy 2014 (PMID:25041788)
Kiryu M et al., Europace 2012 (PMID:22454409)
Hsu SP et al., Atherosclerosis 2011 (PMID:21440892)
Radwitz J et al., Cell Mol Life Sci 2022 (PMID:36309617)
Matsuo R et al., JID Innov 2022 (PMID:36262667)
Lawson CD et al., Elife 2022 (PMID:36039640)
Benedyk TH et al., J Biol Chem 2022 (PMID:36243114)
Tomasz H Benedyk et al., PLoS Pathog 2021 (PMID:34398933)
Gonzalo P Solis et al., Nat Commun 2022 (PMID:35440597)
Mert Duman et al., Biomedicines 2022 (PMID:35740439)
Shadle SC et al., Hum Mol Genet 2019 (PMID:31630170)
Morita A et al., Life (Basel) 2021 (PMID:34685458)
Koinuma S et al., Neurobiol Aging 2021 (PMID:34329965)
Duman M et al., Nat Commun 2020 (PMID:32647127)
Patil AV et al., J Biol Chem 2017 (PMID:28159839)
Guo J et al., Mol Cell Biol 2017 (PMID:28674187)
Beauchemin M et al., Pharmacol Res 2020 (PMID:31874253)
Roesler MK et al., Front Cell Neurosci 2019 (PMID:31474830)
Tang X et al., Sci Rep 2019 (PMID:31641193)
Lombino FL et al., Sci Rep 2019 (PMID:31685876)
Sean C. Shadle et al., bioRxiv 2019 (Epub)
Chew GL et al., Dev Cell 2019 (PMID:31327741)
de Wispelaere M et al., Nat Commun 2019 (PMID:31371704)
Vaqui? A et al., Cell Rep 2019 (PMID:31189102)
Brugger V et al., Nat Commun 2017 (PMID:28139683)
Dong H et al., Sci Rep 2016 (PMID:26846992)
Meng J et al., J Virol 2015 (PMID:26468535)
Chen CW et al., Cell Rep 2016 (PMID:27452458)
Jayaprakash P et al., J Cell Sci 2015 (PMID:25736295)
Rubin B et al., Cancer Invest 2015 (PMID:26536286)
Meng J et al., MBio 2014 (PMID:25249281)
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
，但是进口品牌如Abcam、Millipore、CST等价格偏高，一般在￥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体，分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody （货号E021010 ）是小鼠源的单抗，其效价很高，厂商推荐的WB起始稀释比率为1:1000，实际应用过程中，有使用1:10000或更高的报告，远远高于此稀释比率，说明其免疫效价很高，另外，其检测种属也很多，包括人、牛、猪、小鼠
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated； 货号21337，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号3052，产品描述：Rabbit anti-Human IgG secondary antibody HRP conjugated； 货号300904050，产品描述：One-Hour Western kit，10 ml / bottle x 3；
关于内参，已经有很多的帖子讨论，但是在我们的动物或细胞的模型中，总是有各种干扰的过程常常影响内参的表达，比如GAPDH就参与糖代谢，变性性疾病，凋亡等多种过程，如果我们的试验中存在这种过程干扰内参的表达，应该如何选择呢？Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody Glyseraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme
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