![GAPDH antibody [6C5]](https://img1.dxycdn.com/2023/0711/451/6106825198874858761-14.jpg)
- ￥4300
- GeneTex
- 美国
- GTX28245
- 2025年07月15日
- WB, ICC/IF, IP
- Mouse
- Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rabbit muscle GAPDH
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rabbit
- 目录编号：
GTX28245
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
GAPDH
- 抗体英文名：
GAPDH antibody [6C5]
- 抗体名：
GAPDH 抗体 [6C5]
- 规格：
100 μg
![GAPDH antibody [6C5]](https://img1.dxycdn.com/p/s14/2024/0410/982/4281264461639677771.jpg)
Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A).
Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 oC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
Lane 1: Human GAPDH (1 μg)
Lane 2: GAPDH immunoprecipitated from rat heart tissue extract
Lane 3: Only GTX28245 preincubated with Protein A Sepharose
Lane 4: Only Protein A Sepharose
![GAPDH antibody [6C5]](https://img1.dxycdn.com/p/s14/2024/0410/240/0253273811639677771.jpg)
Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded.
Lane 1: isolated human GAPDH, 0.5 μg
Lane 2: human heart tissue extract
Lane 3: pig heart tissue extract
Lane 4: goat heart tissue extract
Lane 5: bovine heart tissue extract
Lane 6: dog heart tissue extract
Lane 7: mouse heart tissue extract
Lane 8: rat heart tissue extract
Lane 9: rabbit heart tissue extract
![GAPDH antibody [6C5]](https://img1.dxycdn.com/p/s14/2024/0410/614/2304611031639677771.jpg)
Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by:
GTX28245 (green colour)
F-actin microfilaments-bindingdye (red)
DNA-binding dye (dark blue)
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