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30 ul
predicted_size:47.9 kDa
Uniprot ID:P5783
description:KRT18/CK18 mouse monoclonal antibody, clone UMAB50
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul
immunogen:Human recombinant protein fragment corresponding to amino acids 69-372 of human KRT18 (NP_000215) produced in E.coli.
recommended_dilution:IHC 1:100, IF 1:100
predicted_size:47.9 kDa
buffer:PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species:Mouse
applications:IF, IHC, WB
reactivities:Human, Monkey, Mouse, Rat
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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