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100 ul
predicted_size：49.7 kDa
Uniprot ID：Q8WY64
description：MYLIP mouse monoclonal antibody,clone OTI3A2
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]
immunogen：Human recombinant protein fragment corresponding to amino acids 127-385 of human MYLIP(NP_037394) produced in E.coli.
recommended_dilution：WB 1:2000
predicted_size：49.7 kDa
buffer：PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species：Mouse
applications：WB
reactivities：Human, Mouse, Rat
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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