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100 ul
predicted_size：31 kDa
Uniprot ID：Q9NZQ7
description：PD-L1 / CD274 mouse monoclonal antibody, clone OTI2C7 (formerly 2C7)
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：Full length human recombinant protein of human CD274(NP_054862) produced in HEK293T cell.
recommended_dilution：WB 1:200~2000, IF 1:100
predicted_size：31 kDa
buffer：PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species：Mouse
applications：ELISA, FC, IF, LMNX, WB
reactivities：Human (Does not react with: Mouse)
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
population is termed monoclonal . It recognizes a single epitope on the antigenic protein. Antibody producing B lymphocytes can be isolated from the spleen or from lymph nodes. However, they have a finite life span in culture, i.e
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