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- 详细信息
- 技术资料
- 免疫原:
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKC-gamma that contains threonine 655
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX25796
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P, FACS
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
PKC gamma (phospho Thr655)
- 抗体英文名:
PKC gamma (phospho Thr655) antibody
- 抗体名:
PKC gamma (phospho Thr655) 抗体
- 规格:
50 μl
IHC-P analysis of human cerebellum tissue using GTX25796 PKC gamma (phospho Thr655) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
IHC-P analysis of human cerebellum tissue using GTX25796 PKC gamma (phospho Thr655) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB analysis of whole cell extracts (30 μg lysate) of HCT 116 (Lane 1), treated for 20 minutes with 200 mM of PMA (Lane 2), and A549 (Lane 3) treated for 20 minutes with 200 mM of PMA (Lane 4) using GTX25796 PKC gamma (phospho Thr655) antibody.
Dilution : 1:500
WB analysis of samples using GTX25796 PKC gamma (phospho Thr655) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 12) eliminates the signal further verifying that the antibody is phospho-specific.
Dilution : 1:500
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