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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1146, 1150 and 1151 of the human insulin receptor. The corresponding residues in the IGF1R are 1131, 1135 and 1136.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Chicken
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX25681
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-Fr
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
IGF1R (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1146 / Tyr1150 / Tyr1151)
- 抗体英文名:
IGF1R (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1146 / Tyr1150 / Tyr1151) antibody
- 抗体名:
IGF1R (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1146 / Tyr1150 / Tyr1151) 抗体
- 规格:
50 μl
WB analysis of samples using GTX25681 IGF1R (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1146 / Tyr1150 / Tyr1151) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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文献和实验Tramutola A et al., Neurobiol Dis 2020 (PMID:31987911)
Chen YT et al., Oncotarget 2016 (PMID:27322146)
Basso D et al., J Cell Physiol 2011 (PMID:20717964)
Zuliani I et al., Neurotherapeutics 2020 (PMID:33258073)
的关系理不出头绪。GSK-3 beta 总蛋白减少,p-GSK-3 beta (ser 9)增加,是不是足以说明有活性的GSK-3 beta 减少更多,这样理解不知道对不对,希望给以指点。 galaxy22 楼主你好,请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的,active B catenin是减少的,那我有必要做ser9 和tyr216的磷酸化形式吗?我想研究wnt和akt。。。 棉花糖的温柔
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内,每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激,我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤,分别固定,打孔,猝灭,封闭,然后一抗孵育,二抗孵育,最后显色,用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正,最后得出以上数据。Quantitative
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
substrate identified by the two‐hybrid system. Science 257:680‐682. Zhang, H., Zha, X., Tan, Y., Hornbeck, P.V., Mastrangelo, A.J., Alessi, D.R., Pokakiewicz, R.D., and Comb, M.J
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