- ￥4700
- GeneTex
- 美国
- GTX25658
- 2025年07月14日
- WB, IHC-P
- Rabbit
- Human, Rat
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- 详细信息
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCd that contains serine 664.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25658
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
PKC delta (phospho Ser664)
- 抗体英文名：
PKC delta (phospho Ser664) antibody
- 抗体名：
PKC delta (phospho Ser664) 抗体
- 规格：
50 μl
IHC-P analysis of human breast carcinoma tissue using GTX25658 PKC delta (phospho Ser664) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB (peptide competition) analysis of K562 cells stimulated with PMA using GTX25658 PKC delta (phospho Ser664) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptides corresponding to other PKC isoforms (Lane 5-12)control.The data show that the peptide corresponding to PKCdelta (pS664) blocks the antibody signal. The peptide corresponding to PKCalpha (pS657) partially blocks the antibody signal, while those for PKCbetaII (pS660), and rat PKCdelta (pS662) block the antibody signal more thoroughly, indicating their respective reactivity with this antibody. The membrane treated with phosphatase (Lane 13) eliminates the signal further verifying that the antibody is phospho-specific.
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