- ￥5600
- GeneTex
- 美国
- GTX25650
- 2025年07月15日
- WB, ICC/IF, IHC
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1086. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25650
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1086 of mature EGFR is equivalent to Tyr1110 of uncleaved EGFR.
- 抗体英文名：
EGFR (phospho Tyr1086) antibody
- 抗体名：
EGFR (phospho Tyr1086) 抗体
- 规格：
50 μl
WB analysis of 30 μg of A549 (Lane 1) treated for 10 minutes with 200 ng/ml of EGF (Lane 2), A431 (Lane 3) treated for 10 minutes with 200 ng/ml of EGF (Lane 4) cell lysate using GTX25650 EGFR (phospho Tyr1086) antibody.
Dilution : 1:1000
ICC/IF analysis of A431 cells treated with Afatinib (1uM for 6hrs) followed by EGF (200ng/ml for 10 minutes) using GTX25650 EGFR (phospho Tyr1086) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 15 minutes
Dilution : 1:100 in 0.1% BSA incubated at 4ºC
WB analysis of membrane enriched extracts (30 ug lysate) of A-431 (Lane 1) treated with EGF (200ng/ml for 10 minutes) (Lane 2), A-431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200ng/ml for 10 minutes) (Lane 3), A-431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 4), A549 (Lane 5) treated with EGF (200ng/ml for 10 minutes) (Lane 6), and A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 7) using GTX25650 EGFR (phospho Tyr1086) antibody.
Dilution : 1:1000
WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF for 15 minutes (Lane 2-5) using GTX25650 EGFR (phospho Tyr1086) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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文献和实验【求助】干扰一个基因表达后，EGFR的表达及tyr1173磷酸化都降低，如何分析
zxlbaby 干扰一个基因表达后，EGFR的表达及tyr1173磷酸化都降低，如何分析？能说此基因具有调节EGFR的表达和磷酸化作用吗？还是磷酸化减少是因为表达减少？郁闷中。求助。 amygdala 用WB检测以后最好用条带扫描软件做一个量化统计分析，看看总EGFR和Tyr1173降低的比例是否一致，这样就可以知道是哪种情况了。 lilili1979 同意楼上的,可以考虑用磷酸化的/总蛋白
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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