- ￥6300
- GeneTex
- 美国
- GTX60875
- 2025年07月10日
- WB, ICC/IF, ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60875
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
- 抗体英文名：
Histone H3K4me2 (Di-methyl Lys4) antibody [RM135]
- 抗体名：
Histone H3K4me2 (Di-methyl Lys4) 抗体 [RM135]
- 规格：
100 μg
ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60875 Histone H3K4me2 (Di-methyl Lys4) antibody [RM135].
Red : Primary antibody
Green : Actin
WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60875 Histone H3K4me2 (Di-methyl Lys4) antibody [RM135].
Dilution : 0.025μg/ml
The GTX60875 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with mono- methylated Lysine 4 (K4me1), trimethylated Lysine 4(K4me3), or other methylations in histone H3.
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文献和实验," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
control) relative to the level of the signal of interest (ChIP sample). This hampers distinguishing a ''true signal'' from the background signal. Therefore, we recommend setting up ChIP using an antibody against an invariant domain of histone H3 [19,20
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
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