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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX24570
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
IHC-Fr, FACS, IP, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
HLA-G
- 抗体英文名:
HLA-G antibody [MEM-G/11]
- 抗体名:
HLA-G 抗体 [MEM-G/11]
- 规格:
100 μg
Surface staining of JEG-3 human choriocarcinoma cell line with anti-human HLA-G: FITC (GTX78370). Total viable cells were used for analysis.
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文献和实验Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins
in our laboratory to distinguish mRNAs encoding HLA-G5 and HLA-G6, and antibody-based protocols for identification of the soluble isoforms.
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
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