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- 规格:
50 ug
predicted_size:null
Uniprot ID:Q8N3V7
description:Synaptopodin (SYNPO) mouse monoclonal antibody, clone G1D4, Aff - Purified
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
immunogen:Isolated Rat kidney glomeruli.
recommended_dilution:Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (After microwave treatment). Working Dilutions : 1/5-1/10 for 1h at RT. Western blot: 0.1-0.5 µg/ml. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
predicted_size:null
buffer:Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer; State: Aff - Purified; State: Lyophilized purified Ig fraction
host_species:Mouse
applications:IF, IHC, WB
reactivities:Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat
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文献和实验The production of monoclonal antibody by hybridoma
. Keywords: monoclonal antibody ; hybridoma ; fusion Reference 1) Kohler G, and C. Milstein Continuous cultures of fused cells secreting
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
crest development. Dev. Biol. 157:517‐525. Kubota, Y., Morita, T., and Ito, K. 1996. New monoclonal antibody (4E9R) identifies mouse neural crest cells. Dev. Dyn. 206:368‐378
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