GFP antibody [9F9.F9]

Figure: Western blot of GFP recombinant protein detected with GeneTex monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. GTX21218 detects a 27 kDa band corresponding to the epitope tag GFP. The membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDyeR 800 conjugated goat anti-Mouse IgG [H&L]. IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western Blot of anti-GFP monoclonal antibody (GTX21218). Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates). Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4ºC. Secondary antibody: HRP-conjugated anti-Mouse IgG (GTX26728) was performed at a dilution of 1:20,000 for 1h at 4ºC. Block: TTBS supplemented with 1% BSA for 1 h at 4ºC. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.