NLRP3 goat polyclonal antibody

gene_symbol：NLRP3
predicted_size：null
Uniprot ID：Q96P2
description：NLRP3 goat polyclonal antibody, Aff - Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：NLRP3 is a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis.
immunogen：Peptide with sequence from the internal region of the protein sequence according to NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1.
recommended_dilution：Peptide ELISA: 1/64000 (Detection Limit).  Western blot: Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.  Immunohistochemistry on Paraffin Sections: 2-4 µg/ml. In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.
predicted_size：null
buffer：Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified IgG fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
host_species：Goat
applications：ELISA, IHC, WB
reactivities：Bovine, Canine, Human, Mouse, Porcine, Rat